Abstract:
I will show how an in-depth description of the basic principles of diffraction-unlimited fluorescence microscopy has spawned MINFLUX [1-4], a recent superresolution method that has reached the resolution of the size of a fluorophore molecule. Providing 1-3 nanometer resolution in fixed and living cells, as well as localization precisions in the Angstr?m range, MINFLUX and the related MINSTED concept [5,6] are being established for routine applications in the biomedical sciences [4]. Relying on fewer fluorescence photons than other methods, these techniques are also poised to characterize dynamic processes at the single protein level, as already demonstrated by tracking sub(nanometer) details of the unhindered stepping of the motor protein kinesin-1 on microtubules at up to physiological ATP concentrations[7].
[1] Balzarotti, F., Eilers, Y., Gwosch, K. C., Gynn?, A. H., Westphal, V., Stefani, F. D., Elf, J., Hell, S.W. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science 355, 606-612 (2017).
[2] Eilers, Y., Ta, H., Gwosch, K. C., Balzarotti, F., Hell, S. W. MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution. PNAS 115, 6117-6122 (2018).
[3] Gwosch, K. C., Pape, J. K., Balzarotti, F., Hoess, P., Ellenberg, J., Ries, J., Hell, S. W. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Nat. Methods 17, 217–224 (2020).
[4] Schmidt, R., Weihs, T., Wurm, C. A., Jansen, I., Rehman, J., Sahl, S. J., Hell, S. W. (2021) MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope. Nat. Commun. 12:1478.
[5] Weber, M., Leutenegger, M., Stoldt, S., Jakobs, S., Mihaila, T. S., Butkevich, A. N., Hell, S. W. MINSTED fluorescence localization and nanoscopy. Nat. Photon. 15, 361-366 (2021).
[6] Weber, M., von der Emde, H., Leutenegger, M., Gunkel, P., Sambandan, S., Khan, T. A., Keller-Findeisen, J., Cordes, V. C., Hell, S.W. MINSTED nanoscopy enters the ?ngstr?m localization range. Nat. Biotechnol., 41, 569-576 (2023).
[7] Wolff, J.O., Scheiderer, L., Engehard, T., Engelhardt, J., Matthias, J., Hell, S.W. MINFLUX dissects the unimpeded walking of kinesin-1, Science, 379, 1004-1010 (2023).
SPEAKERS / PERFORMERS:
Stefan W. Hell (Nobel Laureate in Chemistry 2014)
Max Planck Institute for Multidisciplinary Sciences, G?ttingen & Max Planck Institute for Medical Research, Heidelberg
Stefan W. Hell is a director at both the Max Planck Institute for Multidisciplinary Sciences in G?ttingen, and the Max Planck Institute for Medical Research Heidelberg, Germany. Stefan W. Hell received his doctorate (1990) in physics from the University of Heidelberg. From 1991 to 1993 he worked at the European Molecular Biology Laboratory and followed with stays as a senior researcher at the University of Turku, Finland, between 1993 and 1996, and as a visiting scientist at the University of Oxford, England, in 1994. In 1997 he was appointed to the MPI for Biophysical Chemistry in G?ttingen as a group leader and was promoted to director in 2002. From 2003 to 2017 he also led a research group at the German Cancer Research Center (DKFZ). Hell holds honorary professorships in physics at the Universities of Heidelberg and G?ttingen. Stefan W. Hell is credited with having conceived, validated and applied the first viable concept for overcoming Abbe’s diffraction-limited resolution barrier in a light-focusing fluorescence microscope. For this accomplishment he has received numerous awards, including the 2014 Kavli Prize in Nanoscience and the Nobel Prize in Chemistry. In 2022 Hell was admitted to the “Order Pour le Mérite". In 2023 he received the honorary medal "In Publica Commoda" of the University of G?ttingen and was awarded the Werner-von-Siemens-Ring.
Language:
English
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